- Pluripotent stem cell-derived organoids: using principles of developmental biology to grow human tissues in a dish
Summary: This Development at a Glance article summarises how knowledge gained from developmental biology can be used to guide human in vitro organogenesis, and discusses the potential applications of this technology.
- Translational applications of adult stem cell-derived organoids
Summary: This Primer article discusses how adult stem cell-derived organoids can be used to model human diseases, create personalized cancer therapies and further efforts in regenerative medicine.
- Embryoids, organoids and gastruloids: new approaches to understanding embryogenesis
Summary: This Review article discusses the basic physical and biological principles that underlie the self-organization of embryonic stem cells into organoids, and how this informs human development.
- Lung organoids: current uses and future promise
Summary: This Review article explores the latest advances in both adult and embryonic stem cell-derived lung organoid culture, and discusses how these systems can be used to understand homeostasis and regeneration.
- Dissecting the stem cell niche with organoid models: an engineering-based approach
Summary: This Review article discusses how organoids have been used to model and characterize stem cell-niche interactions and how new engineering approaches enable systematic study of the stem cell niche.
- Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells
Summary: Co-differentiation of endothelial cells and cardiomyocytes from human pluripotent stem cells provides a cardiac microtissue model with potential applications for disease modelling and drug discovery.
- Intestinal epithelial organoids fuse to form self-organizing tubes in floating collagen gels
Summary: Culturing conventional mouse intestinal organoid cultures in contracting collagen gel induces the formation of macroscopic tubes, with all major cell types and crypt- and villus-like domains.
- Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Summary: Optimised strategies and protocols enable a range of complex genetic manipulations directly in mouse and human neural stem cell lines.
- Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells
Summary: Paracrine signals from MSCs or HUVECs are able to promote hepatocyte differentiation independently, but both must co-exist to allow for the cell-cell contact and organization into a 3D liver organoid.
- Development of a human cardiac organoid injury model reveals innate regenerative potential
Summary: Human cardiac organoids, which represent immature tissue, show an innate regenerative response and robust recovery after cryoinjury without pathological fibrosis or hypertrophy.