- Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells
Summary: Optimised strategies and protocols enable a range of complex genetic manipulations directly in mouse and human neural stem cell lines.
- Formative pluripotency: the executive phase in a developmental continuum
Summary: This Hypothesis article poses that a third state of pluripotency, called formative pluripotency, exists between the naïve and primed states, and is enabling for the execution of pluripotency.
- Sequential organogenesis sets two parallel sensory lines in medaka
Summary: Two parallel sensory lines in medaka share a common origin and are composed of identical organs that are, nevertheless, generated through different morphogenetic programmes.
- Efficient TALEN-mediated gene targeting of chicken primordial germ cells
Summary: TALE nucleases are used to target the DDX4 (vasa) locus in chicken primordial germ cells and generate DDX4 knockouts, which provide insights into DDX4 function in early chick development.
- Plant regeneration: cellular origins and molecular mechanisms
Summary: This Review article summarises how plants control various types of regeneration and discusses how developmental and environmental constraints influence these regulatory mechanisms.
- Jmjd2c facilitates the assembly of essential enhancer-protein complexes at the onset of embryonic stem cell differentiation
- Neural tube closure: cellular, molecular and biomechanical mechanisms
Summary: This Review discusses the cellular, molecular and biomechanical mechanisms involved in neural tube closure, focusing on the most recent advances in the field.
- Brassinosteroid signaling directs formative cell divisions and protophloem differentiation in Arabidopsis root meristems
Highlighted article: In addition to controlling cell expansion, brassinosteroid signaling acts cell-autonomously and non-cell-autonomously to promote protophloem differentiation and restrict formative divisions in root meristems.
- Integrins are required for tissue organization and restriction of neurogenesis in regenerating planarians