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Figure 6


Fig. 6. Expression of notochord and nerve cord markers in two-celled partial embryos treated with bFGF. (A) Diagram to show the experimental procedure to make two-celled partial embryos treated with bFGF. (B,D,F) Expression of tissue-specific markers in two-celled partial embryos treated with bFGF. (B) Expression of Not-1 antigen. Both presumptive notochord blastomere (larger blastomere) and presumptive nerve cord blastomere (smaller one) show strong Not-1 expression. (D,F) Expression of HrETR-1 (D) and HrTBB2 (F) is not seen in either blastomere. (C,E,G) Expression of tissue-specific markers in two-celled partial embryos without FGF treatment. (C) Not-1 antigen is not expressed in either blastomere. (E,G) Both blastomeres show HrETR-1 (E) and HrTBB2 (G) expression. Scale bar: 100 µm.