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Fig. 4. TUNEL staining of cultured primary chondrocytes. Primary chondrocytes from wild type (WT), Stat1-/-, TgFGF and TgFGF/Stat1-/- mice were isolated from P10 growth plate and plated at similar density. After 24 hours in the culture, the cells were fixed and stained for TUNEL. (A) Hoechst staining visualizing the nuclei of the cells; (B) TUNEL-positive cells in the same field as A.