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Fig. 2. Reporter gene expression patterns in the wing and haltere imaginal discs. (A) MCP822 construct containing the wild-type element. lacZ expression is correctly maintained: it is expressed in the posterior compartment of the haltere disc (ps6) but is silenced in the wing disc (ps4 and ps5) and in the anterior compartment of the haltere disc (ps5). (B) 5MPHO construct in which all the putative PHO binding sites are mutated. The expression of transformant line T4 is shown. lacZ is strongly derepressed in the wing and haltere discs. (C) MCP1 construct carrying the fragment from residues 573 to 675. lacZ is derepressed in the wing and haltere discs. (D) 5MPHO line T8, in which lacZ is weakly derepressed. (E) {Delta}5MPHO: a derivative line of 5MPHO line T8 from which 5MPHO had been excised. Expression is strongly derepressed in wing and haltere discs.