Fig. 2. Expression of Hoxd10 in normal chick embryos at stage 29. (A) Ventral view of posterior spinal regions after whole-mount in situ hybridization. (B-E) Transverse sections through T7 (B), LS1 (C), LS2 (D), and LS3 (E) after section in situ processing. Hoxd10 is highly expressed in the LS spinal cord, sensory ganglia and spinal nerves (A). Expression is graded on the AP axis, being lowest in LS1 and highest in middle and posterior LS segments (A, C-E). Within LS1 and LS2, two dark strips of Hoxd10 expression are visible in A. In transverse sections (C,D), these strips represent high expression in cells at the lateral edge of the LMC and in intermediate cord regions. Cells within middle and dorsomedial motor column regions show less expression (arrow, E). Slight Hoxd10 expression is visible in T7 but not in more anterior T segments (A,B). Bars, 250 µm.