Development -- Mead et al. 128 (12): 2301 Figure IG6

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Fig. 6. Ectopic expression of XLMO-2 in FGF-treated animal caps induces globin gene expression. (Top) Animal pole explants were harvested from embryos injected with XLMO-2 mRNA, treated with 20 ng/ml bFGF and allowed to develop to stage 33. (Bottom) RT-PCR analysis was performed for tadpole ${alpha}$ -globin and SCL. XLMO-2 expression in FGF-treated animal caps leads to expression of ${alpha}$ -globin and SCL. EF-1 ${alpha}$ was included as a control for RNA recovery. Embryo lane is a whole sibling embryo used as a positive control for the RT-PCR procedure; –RT is a standard reaction that lacks reverse transcriptase and is included as a control for genomic DNA contamination.