Fig. 2. mShhN specifies neurons in E11 rat telencephalic explants expressing Dlx, Mash1 and/or Islet 1/2, similar to those found in vivo. mShhN (1 µg/ml) -treated explants (A,B,E,F,I,J,M,N), uShhN (1 µg/ml) -treated explants (C,G,K,O), untreated control (-; D,H,L,P). (A-D) Dlx (green), Mash1 (red), and Dlx and Mash1 co-expressing cells (yellow). (E-H) Dlx (green), Nkx2.1 (red). (I-L) Dlx (green), Islet 1/2 (red), and Dlx and Islet 1/2 (yellow), (M-P) Islet 1/2 (green), Mash1 (red), and Islet 1/2 and Mash1 (yellow).