Fig. 7. (A) Western analysis of recombinant and virally produced Shh proteins. Recombinant proteins or lysates were loaded onto a 12.5% SDS-PAGE gel, western blotted using 5E1 (anti-Shh antibody) and visualized using a chemiluminescence substrate (NEN). Recombinant proteins (1 µg): 1, C24S-ShhN; 2, mShhN; 3, uShhN; 4 and 5, lysates from virally infected cells (4, C24S-Shh virally infected cell lysate; 5, wild-type Shh virally infected cell lysate. Extract in 4 was immunoprecipitated first and then immunoblotted; extract in 5 was acetone-precipitated first and then immunoblotted. Prestained markers are shown on the left. (B) Schematic of recombinant and virally produced Shh proteins, and their activities.