Fig. 8 The C24S-ShhN protein is equivalent to uShhN in its ability to induce ventral telencephalic neurons expressing Dlx and Islet1/2 in forebrain explants. Rat E11 telencephalic explants were treated with different concentrations of uShhN or C24S-ShhN and stained with anti-Dlx and anti-Islet 1/2 antibodies. The same region of E11 rat telencephalic explant was used for ventral neural induction as in Fig. 3. The level of induction is indicated by the number of cells expressing Dlx (green), Islet 1/2 (red) or both (yellow). Explants were treated with uShhN at the following concentrations: A, 960 nM; C, 386 nM; E, 48 nM. Or with C24S-ShhN: B, 960 nM; D, 386 nM; F, 48 nM (n=4).