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Fig. 9. Immunoprecipitation of radiolabeled Shh (arrow) from the mouse neural cell line C17. 3H-palmitate + 3H-myristate incorporation (lanes 1-4). Lane 1: cytoplasmic fraction from control C17 cell line. Lane 2: cytoplasmic fraction from Shh transfected C17 cell line (Shh-C17). Lane 3: 125,000 g pellet, membrane fraction from control C17 cell line. Lane 4: 125,000 g pellet fraction from Shh-C17. 3H-myristate incorporation (lanes 5,6). Lane 5: 125,000 g pellet fraction from Shh-C17. Lane 6: culture supernatant from Shh-C17. 3H-palmitate incorporation (lanes 7,8). Lane 7: 125,000 g pellet fraction from Shh-C17. Lane 8: culture supernatant from Shh-C17. 35S-methionine+cysteine incorporation (lanes 9-14). Lane 9: cytoplasmic fraction from control C17 cell line. Lane 10: cytoplasmic fraction from Shh transfected C17 cell line (Shh-C17). Lane 11: 125,000 g pellet, membrane fraction from control C17 cell line. Lane 12: 125,000 g pellet fraction from Shh-C17. Lane 13: culture supernatant from control C17 cell line. Lane 14: culture supernatant from Shh-C17 (secreted form).