Fig. 5. Foregut and sclerotome expression of Foxf1 depends on Shh. Foxf1 whole-mount in situ hybridization of wild-type (A-D) and Shh^-/- (E-H) E10.5 (A,B,E,F) or E12.5 (C,G) embryos and E12.5 lungs (D,H). In Shh mutants, Foxf1 expression is missing in the anterior part of the gut, the oral cavity, in sclerotomes and lungs. Foxf1 mRNA persists only in the posterior part of the gut, where Ihh is expressed. Embryos in A,E are in aqueous solution, to visualize the general morphology. Embryos in B,C,F,G are clarified in benzylalcohol:benzylacetate to show staining of internal structures. In, intestine; Li, liver; Lu, lung; OC, oral cavity; Sc, sclerotome.