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Fig. 7. Loss of ventrolateral mesoderm and expansion of neural territories in embryos that lack vox and vent function. Expression of pax2.1 (A-D), gata1 (E-H), nkx2.5 (I-M) and krox20 (N-Q) was examined by in situ hybridization at the (A-D,N-Q) three-somite stage (11 hours) or (E-M) 9-10 somite stage (~14 hours). (A,E,I,N) Wild type. (B,F,J,O) Dfst7 mutants. (C,G,K,P) Wild-type embryos injected with vox MO. (D,H,L,M,Q) Wild-type embryos co-injected with vox and vent MO. (A-D) pax2.1 is expressed in the pronephros and pronephric duct (arrowheads in wild-type embryos) and at the mid-hindbrain junction. (K-M) Arrowheads mark expression of nkx2.5. (A-J) Dorsal views, anterior towards the left. (K,L,N-Q) Lateral views, anterior towards the left. (M) Posterior view, dorsal towards the top.