Fig. 9. Interactions among vox, vent, chordin and bozozok. The genotype of the embryos is shown at the top of the panel, and the use of MO, when injected, is shown at the bottom of the panel. The gene analyzed by in situ hybridization is shown on the left side of each row. Embryos were analyzed at 75% epiboly (A-H), 40% epiboly (I-L), or 50% epiboly (M-P). In boz mutant embryos co-injected with vox and vent MO, gsc expression resembled that shown in P (data not shown). In Df^st7;chd double mutants, domains of gata2 (D) and gsc (L) expression are both expanded when compared with wild type (A,I). In each of several cases examined, gata2 expression was slightly less expanded in Df^st7; chd mutants (D) than in chd single mutants (C); a similar result was observed in comparing chd mutants injected with vox and vent MO (H) and chd mutants injected with vox MO (G). The reduced expansion of the ventral marker gata2 (D,H) correlates with the increase in gsc expression in chd mutants lacking vox and vent function (L). (A-D,I-P) Animal pole views, dorsal towards the right. (E-H) Lateral views, dorsal towards the right. In A-D,I-P, genotypes were determined by PCR assay after the photographs were taken.