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Fig. 2. Characterization of diencephalic defects with molecular markers. (A,C,E,G,I,K,M,O,Q) Wild-type and (B,D,F,H,J,L,N,P,R) Emx2-/-Otx2+/- embryos. (A,B) Tcf4, (C,D) Lim1, (E,F) Dlx1, (G,H) Gbx2, (I,J) Ebf1, (K,L) Pax6, (M,N) Otx1, (O,P) Mek4 and (Q,R) ephrin-A2 expressions. (C,D) Whole-mount hybridization of 10.5 dpc embryos; the rest are in situ hybridization of sagittal sections of 11.5 dpc embryos. apt, anterior pretectum; at, anterior tectum; dt, dorsal thalamus; ge, ganglionic eminence; ht, hypothalamus; ppt, posterior pretectum; pt, pretectum; tg, tegmentum; vt, ventral thalamus; zl, zona limitans. Scale bars: 125 µm.