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Fig. 4. Characterization of double mutant defects with molecular markers at six-somite stage. (A) Otx2+/-, (C,E,G,I,K,M) wild-type and (B,D,F,H,J,L,N) Emx2-/-Otx2+/- embryos. (A,B) Otx2, (C,D) Pax6, (E,F) Wnt1, (G,H) En1, (I,J) Pax5, (K,L) Fgf8 and (M,N) Six3 expression. The Wnt1-negative r1/2 is indicated by arrowheads in E,F. Arrow in K indicates the concentration of the Fgf8 expression into future isthmus at this stage. Asterisks indicate the optic cup. Scale bars: 150 µm.