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Fig. 2. Treatments with PKI{alpha} antisense oligonucleotides interfere with the proper formation of L-R axis. S, sense; AS, antisense. (A,B) Examples of heart situs (indicated by red arrowheads) in embryos treated with S (A) and AS (B) oligonucleotides. Ventral views are shown. AS treatment caused reversed heart looping in some embryos. (C,D) Examples of Shh expression in embryos treated with S (C) and AS (D) oligonucleotides. Dorsal views are shown. AS treatment did not change the asymmetric expression of Shh. (E,F,I,J) Examples of Nodal expression in embryos treated with S (E,I) and AS (F,J) oligonucleotides. Dorsal views (E,F) and coronal sections (I,J) are shown. AS treatment induced bilateral expression of Nodal. (G,H,K,L) Examples of Pitx2 expression in embryos treated with S (G,K) and AS (H,L) oligonucleotides. Ventral views (G,H) and coronal sections (K,L) are shown. AS treatment induced bilateral expression of Pitx2. (M,N) Examples of PKI{alpha} expression in embryos treated with S (M) and AS (N) oligonucleotides. Dorsal views are shown. AS treatment suppressed the expression of PKI{alpha}, while S treatment did not affect PKI{alpha} mRNA expression. Black arrowheads point to areas of high expression, and white arrowheads point to areas of low expression.