Fig. 2. Regulation of FoxD3 expression in animal cap assay. Animal caps were prepared from stage 10.5 embryos that had been injected with (A,B) control mRNA (100 pg), (C,D) Chd mRNA (50 pg), (E,F) Wnt3a mRNA (50 pg), (G,H) Chd and Wnt3a mRNAs, (I,J) Slug mRNA (50 pg), (K,L) Slug and Wnt3a mRNAs, and (M,N) Zic-r1 mRNA (100 pg). The animal caps were harvested at stage 17 and analyzed with FoxD3 probe (A,C,E,G,I,K,M) or Slug 3'UTR probe (B,D,F,H,J,L,N).