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Fig. 2. Whole-mount in situ hybridization analysis. (A) Aristaless. (B) Fibrinogen-related protein (FRP). (C) MSP130-like. (D) C-lectin. (E) NET-7. (F) ERG. (G) Fibronectin. (H) SM50-related. Expression of aristaless, MSP130-like, SM50-related and C-lectin is restricted to PMCs at the mesenchyme blastula stage. FRP is expressed by PMCs and by ectoderm cells at the animal pole. ERG mRNA is expressed by PMCs and non-skeletogenic mesoderm cells in the vegetal plate. At the late gastrula stage, fibronectin mRNA is enriched in PMCs (arrow, G).