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Fig. 4. BMP-4 induced notochord in the primary lineage. Immunofluorescence for Not-1 antigen. (A) Notochord precursors isolated at the 24-cell stage did not form notochord. (B) Treatment with BMP-4 induced notochord formation. (C) U0126 blocked notochord induction by BMP-4. Scale bar: 50 µm.