Fig. 6. Sxl Co-immunoprecipitates with Fu, Cos2 and -tubulin. (A) Sxl was immunoprecipitated from wild-type ovaries, Sxlf4 ovaries and Sxl null (SxlfP7BO) males. 10% of the post-immunoprecipitation lysate and all of the immunoprecipitates were analyzed by Western blot with anti-Fu, anti-Cos2 or anti--tubulin antibodies. Fu, Cos2 and -tubulin co-immunoprecipitates with Sxl in Sxlf4 and wild-type ovaries. The percent of protein that immunoprecipitated with Sxl in wild type and Sxlf4 ovaries was, respectively, 13.6 and 15.1% for Cos2, 8.8 and 10.6% for Fu, and 10.6 and 10.2% for -tubulin. The same blots were probed with Fu, Cos2, -tubulin and Sxl antibodies but the Cos2 signal in the Sxlf4 immunoprecipitates is a repeat immunoprecipitate for a cleaner signal. Immunoprecipitates were performed at least twice. (B) BicD was immunoprecipitated from wild-type ovaries and probed with Fu and -tubulin antibodies. -tubulin was immunoprecipitated from Sxlf4 ovaries and probed with anti-Sxl antibodies.