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Fig. 11. (A-D) Expression pattern of CsASH1 and CsASH2 in the PNS. Whole mounts; anterior is towards the top. (A) At 200 hours, CsASH1 is expressed in four clusters of cells each in the pedipalps and the legs (arrows). (B) At the same time, CsASH2 is also expressed in the PNS in non overlapping cell clusters (arrows). (C) At 220 hours, CsASH1 expression in the PNS disappears. There is only a weak staining left at the base of the extremities. (D) At the same time, CsASH2 is expressed in 11 new cell clusters (arrows). Scale bar: 200 µm.