Fig. 13. (A-D) Phenotypic analysis of embryos stained with an antibody against HRP after injection of CsASH1 dsRNA; anterior is towards the top. (A,D) The prosoma and opisthosoma, respectively, of an embryo injected with GFP dsRNA as a control. The embryo shows the normal HRP staining. The arrows point to staining in the PNS. (B) Prosoma of an embryo injected with CsASH1 dsRNA. Several segments of the prosoma show no HRP staining (arrows). (C) Prosoma of an embryo injected with CsASH1 dsRNA. This embryo shows an asymmetric effect: only one half of the germ band shows a reduced staining. (E) Opisthosoma of an embryo injected with CsASH1 dsRNA. All invagination sites are missing in the opisthosoma. (F) Opisthosoma of an embryo injected with CsASH1 dsRNA. Invagination sites are reduced in the opisthosoma. Only the anterior-most lateral invagination sites are visible (arrow). Ch, cheliceral segment; ped, pedipalpal segment; l1 to l4, walking legs 1 to 4 (corresponding to prosomal segments 3 to 6); o1 to o4, opisthosomal segments 1 to 4. Scale bar: 200 µm.