Fig. 8. (A,B) Deduced amino acid sequence of the bHLH domain of CsASH1 (A) and CsASH2 (B), and their relationship to other bHLH proteins. The bHLH domain is indicated. The alignment compares the basic domain and the two helices of CsASH1 and CsASH2 with three members of the ASC family of Drosophila and with the genes from other species that show the highest identity to the proneural genes of the spider. CsASH1 shows the highest identity to the chicken proneural protein CASH-1, whereas CsASH2 is highly identical to the proneural protein XASH1 of Xenopus. The loop, which varies in length and in sequence, is not included in the percent amino acid identity calculation. Dashes indicate amino acid identity with CsASH1 and CsASH2, and dots indicate gaps in the protein alignment. GenBank Accession Numbers, AJ309490 and AJ309491.