Fig. 2. Selective expression of Nkx2.2 in isolated A2B5 glial progenitor cells, but not in dissociated neurons or astrocytes. (A-C) Immortalized GRP cells were stained simultaneously with DAPI for labeling nuclei (A) and with an antibody against Nkx2.2 (B). A superimposed image (C) revealed that a subset of A2B5 cells express Nkx2.2. (D) Immunopurified A2B5 cells were co-stained with DAPI, anti-Nkx2.2 and A2B5. Again, a subpopulation of cells express Nkx2.2 (arrows). (E,F) Cultured cells from dissociated E13.5 rat spinal cord were labeled simultaneously with DAPI, anti-Nkx2.2 and anti-ßIII tubulin (E) or anti-GFAP (F). Nkx2.2 staining (arrows) is not found in ßIII tubulin-positive neurons or GFAP-positive astrocytes.