Fig. 1. A map of the fry gene. A 50 kb segment is shown. The BDGP/Celera collaboration has predicted seven genes in this region. Three of these (CG6774, CG14171 and CG6780) largely comprise the fry gene. The other four are transcribed on the complementary strand and have not been studied. The relative location and size of the 28 fry exons are shown. cDNA clones have been found in two regions. One group of cDNA clones was near the 5' end of the transcript. The longest of these was about 4 kb and it included sequences from exons 3-14. A second cDNA was found as an EST (SD10447) and is located at the 3' end of the gene. Southern blot analysis and DNA sequencing were used to determine the locations of three fry mutations. The P insertion responsible for fry⁶ was inserted into the first intron of fry. The fry¹ mutation is a frameshift mutation in exon 3. The fry² mutation is a nonsense mutation in exon 22.