Fig. 2. aub mRNA and GFP-Aub expression pattern during oogenesis. (A) In situ hybridization for aub mRNA in a stage 10 egg chamber, showing expression in the nurse cells and oocyte but no localization to the posterior ooplasm. (B) A stage 8 egg chamber displaying cytoplasmic GFP-Aub in the nurse cells, identifiable by their large unstained nuclei and oocyte (arrow). Note that the nurse cell nuclei are closely surrounded by concentrated GFP-Aub (see also Fig. 5). Levels of GFP-Aub are commonly unequal in different nurse cells, as seen here. (C,D) GFP-Aub (green) and Osk (red) at the posterior pole of the oocyte in a stage 10 egg chamber. (E) Merged image of green and red channels showing colocalization (yellow).