Fig. 7. The DNA-binding domain of EGL-38 specifically binds the lin-48 lre2 site with high affinity. E. coli lysate containing EGL-38 DBD was incubated with the lre2 probe at a final dilution of 1:51,200 (top), or with the CD19 control probe at 1:6400 (bottom). Competitor sequences are described in Materials and Methods. The CD19 probe corresponds to the CD19-1 element of Fig. 5.