Development -- Boyl et al. 128 (15): 2989 Figure IG4

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Fig. 4. OTX2 protein level in Otx2 ${lambda}$ mutant embryos. (A) Western blot analysis of 7.5 d.p.c. wild-type, Otx2+/-, Otx2 ${lambda}$ /Otx2 ${lambda}$ and Otx2 ${lambda}$ /- embryos, showing that in Otx2 ${lambda}$ mutants the OTX2 protein is remarkably reduced. (B) Coomassie Blue staining of the same gel in A. (C) Graphic representation of densitometric scanning indicating a reduction of $~$ 60% of OTX2 protein in Otx2 ${lambda}$ /Otx2 ${lambda}$ and Otx2 ${lambda}$ /- mutants when compared with wild-type and Otx2+/- embryos, respectively.