Development -- Boyl et al. 128 (15): 2989 Figure IG5

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Fig. 5. Quantitative analysis and ribosome affinity profiles of Otx2 ${lambda}$ mRNA. (A) Total, cytoplasmic and nuclear Otx2 ${lambda}$ mRNA exhibits a reduction of $~$ 20% when compared with the wild-type Otx2 mRNA. The Otx2 ${lambda}$ mRNA percentages are the mean of three independent experiments. (B) Ribosome affinity profiles of Otx2, ß-actin mRNAs and in vitro synthesised Otx2 ${lambda}$ RNA, showing the distribution of the untranslated Otx2 ${lambda}$ RNA (see also Materials and Methods). (C,D) Ribosome affinity profiles comparing Otx2 ${lambda}$ , Otx2 and ß-actin mRNAs in Otx2 ${lambda}$ /Otx2 embryos at 7.75 (C) and 10.5 d.p.c. (D) show that while more than 50% of the ß-actin and Otx2 mRNAs is detected in the first two fractions, only $~$ 25% of the Otx2 ${lambda}$ mRNA is concentrated in the same fractions. Note that mRNA profiles are not influenced by any quantitative variation of the mRNAs analysed.