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Fig. 3. Increased aggregation of membrane-coated microspheres in a solution containing hyaluronan and a mixture of CSPGs. (A) Control: Membrane-coated microspheres after 30 minutes incubation in 0.1 M PB. (B) Membrane-coated microspheres after 30 min of incubation in 500 µg/ml hyaluronan in 0.1 M PB. (C) Membrane-coated microspheres after 30 minutes incubation in 0.1 M PB containing 100 µg/ml of a CSPG mixture. (D) Membrane-coated microspheres after 30 minutes incubation in 0.1 M PB containing 500 µg/ml hyaluronan and 100 µg/ml of a CSPG mixture. Note the increased number of aggregated microspheres.