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Fig. 1. Characterization of recombinant proteins consisting of EphB receptor extracellular domains fused to the immunoglobulin Fc{gamma} domain. (A) Polyacrylamide gel electrophoresis and silver staining of protein A column-purified EphB1•ECD-Fc (lane 1), EphB2•ECD-Fc (lane 2), EphB3•ECD-Fc (lane 3), and Fc control protein (lane 4). (B-E) Binding of EphB1•ECD-Fc (B), EphB2•ECD-Fc (C), EphB3•ECD-Fc (D) and Fc control (E) onto E14 embryonic retinal axons and growth cones in culture, visualized with Cy3-conjugated anti-Fc antibody. No binding of the Fc control protein (E) was detected. Scale bar, 10 µm.