Fig. 7. Homotopic transplants show that non-migrating CNC cells adopt a neural fate. (A) Non-migrating transplant overexpressing cXcad-11 stained with 2G9 antibody, a neural marker. (B) Migrating GFP-Myc-expressing donor CNC cells (green) are negative for 2G9 (red). (C) Whole-mount in situ hybridisation of a cXcad-11-expressing graft shows that sox3 is not expressed 1 hour post grafting. (D) GFP-Myc immunostaining of the section shown in C. (E) In situ hybridisation with nrp-1 probe 18 hours after grafting. Donor embryo was injected with cXcad-11 RNA. (F) GFP-Myc immunostaining of the section shown in E. Upper half of the transplant is nrp-1 positive. (G) In situ hybridisation with twist probe 18 hours after grafting. Donor embryo was co-injected with cXcad-11 and ß-catenin RNA. (H) Section shown in G immunostained with 2G9 (red). Donor embryos were injected either with 1 ng Xcad-11, 2.5 ng cXcad-11 or 2.5 ng cXcad-11 plus 80 pg ß-catenin RNA. Asterisks mark the graft centres. Scale bars: 50 µm.