Development -- Borchers et al. 128 (16): 3049 Figure IG8

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)

Fig. 8. Non-migrating CNC cells also adopt neural fate after heterotopic transplantation into the pharyngeal pouch area. (A) Xcad-11-expressing transplant was found to be twist negative in whole-mount in situ hybridisation but (B) positive for 2G9 neural marker expression in immunostaining. (C) Transplant from a donor co-injected with ${Delta}$ cXcad-11 and ß-catenin RNA was twist negative in whole-mount in situ hybridisation but (D) positive for 2G9. (E) Transplant from a donor co-injected with ${Delta}$ cXcad-11 and ß-catenin RNA shows nrp-1 expression in whole-mount in situ hybridisation. (F) Higher magnification of the transplant seen in E. (G) GFP-Myc immunostaining of the section shown in F. (H) N-Tubulin-positive transplant from a donor injected with ${Delta}$ cXcad-11 RNA. (I) GFP-Myc immunostaining of the section shown in H. (J) NeuroD-positive transplant from a donor injected with ${Delta}$ cXcad-11 RNA. (K) GFP-Myc immunostaining of the section shown in J. Donor embryos were injected either with 1 ng Xcad-11, 2.5 ng ${Delta}$ cXcad-11 or 2.5 ng ${Delta}$ cXcad-11 plus 80 pg ß-catenin RNA. Scale bars: 50 µm.