Fig. 3. med-6 is involved in regulating genes in the Ras pathway: vulval phenotypes before and after med-6 RNAi in various genetic backgrounds. The left panels show the vulval tissues at the L4 stage before med-6 RNAi, and the right panels, after RNAi. (A,B) lin-3 (e1417); (C,D) lin-3 (syIs1); (E,F) let-23(sa62); and (G,H), let-60(n1700). This figure shows representative animals only. For example, (A) shows a wild-type vulva of an animal of the genotype lin-3(e1417), whose typical phenotype is 70-90% vulvaless, with only 10-30% wild-type vulval induction. For detailed data, see Table 1. The arrows indicate vulval tissue invagination. Wild-type animals always have only one invagination. Scale bar: 25 µm.