Fig. 5. Kidney development in Sall1-deficient mice. (A) Metanephros in wild-type mice at 11.5 dpc. Ureteric bud (ub) branches from Wolffian duct (W) and metanephric mesenchyme (mm) are condensed around the bulging ureteric bud. (B) Metanephros in Sall1-deficient mice at 11.5 dpc. Metanephric mesenchyme is formed but reduced in size and is not invaded by the ureteric bud. (C) Metanephros in Sall1-deficient mice at 11.5 dpc. The left ureteric bud invades the mesenchyme, but does not bulge. (D) Metanephros in wild-type mice at 12.5 dpc. Branching of ureter is evident. (E) Metanephros in Sall1-deficient mice at 12.5 dpc. No kidney is detected. (F) Metanephros in Sall1-deficient mice at 12.5 dpc. Ureteric bud is observed in the left primordium, but no branching occurs. (G) Metanephros in Sall1-deficient mice at 14.5 dpc (k). Cortex and medullar regions are formed and glomeruli are observed. (H) Metanephros in Sall1-deficient mice at 14.5 dpc. Kidney is absent though gonads (g) are present. (I) Metanephros in Sall1-deficient mice at 14.5 dpc. A small kidney with poorly branched tubules and ducts is observed on the right. (J) TUNEL analysis of metanephros in wild-type mice at 11.5 dpc. TUNEL analysis of metanephros in Sall1-deficient mice at 11.5 dpc. Note the yellow apoptotic cells in the mesenchyme. Scale bars: 100 µm