Fig. 2. Growth factors induce branching morphogenesis in mammary organoids. Primary
mammary organoids were embedded in collagen I gels and treated for 4 to 6 days
with EGF, KGF, bFGF or HGF at a concentration of 50 ng/ml. (A) Quantification
of the percentage of branching organoids for each of the treatments. The
means±s.d. of three independent experiments are shown. The differences
between the control group (no GF) and the growth factor-treated groups was
statistically significant (**P
0.01). (B) Appearance of
control and growth factor-treated primary mammary organoids. (C) Cross
sections of organoids showing the existence of a lumen at the day of
preparation (day 0) and after 4 days of treatment with KGF. Scale bars: 200
µm in B; 100 µm in C.