Fig. 2. Pax6 expression in differentiating granule cells during development. (A-D) In situ hybridization with a Pax6 probe was performed on sagittal cryosections of developing rat cerebella of E20 and postnatal days 5, 10 and 16. Pax6 is continuously expressed in developing granule cells in the EGL and the IGL. (E) Higher magnification of P10 cerebellum. Expression is stronger in the EGL than in the IGL. Some cells in the ML also express Pax6 mRNA (arrows). (F) Immunohistochemistry of P10 cerebellum for Pax6 protein reveals intense expression in the EGL and in migrating granule cells with fusiform nuclei in the ML (arrows); expression in the IGL is relatively weak. Scale bars: 250 µm in A-D; 25 µm in E,F.