Fig. 5. Cell-autonomous effect of Pax6 on elongation of bipolar parallel fibers. EGL cells in microexplant cultures were transfected with either GFP cDNA alone (wt, rSey2) or GFP with Pax6 or Pax6-EnR cDNAs (rSey2+Pax6, wt+Pax6-EnR). (A) Morphology of granule cells shown by GFP fluorescence (green) together with immunofluorescence with Zic1 (red) and Pax6 (blue). Scale bar: 20 µm. (B) The length (left) and the number (right) of neurites quantified in GFP expressing cells. Each column in the right graph represents the number of neurites as indicated. (^*P<0.001, ^**P<0.0001, in comparison with the wild-type GFP control; ^***P<0.07 in comparison with the mutant GFP control).