Fig. 8. Functional interaction of Pax6 with the Rho/ROCK pathway in axonogenesis of the granule cell. (A-F) Morphology of neurons from wild-type (A-C) and Pax6 mutant (D-F) EGLs. Dissociated EGL cells were labeled with PKH26 and cultured at a low density for 20 hours. Most wild-type cells (A) have bipolar processes (arrows), whereas excess neurites are present in Pax6 mutant cells (D) as well as Y-27632-treated cells (B) (arrowheads). See text for details. Scale bar: 20 µm. (G,H) Quantitative analyses of the number (G) and length (H) of axons induced by Y-27632-treatment or by C3 misexpression. Data are indicated as mean±s.e.m. P<0.001 in comparison with the respective untreated controls for wild-type cells (white bars) and mutant cells (gray bars).