Fig. 8. Genetic interactions between da and twist. (A-C) Wild-type embryos; (D-F) Embryos expressing one copy of UAS-twist (G-I) or embryos expressing two copies of UAS-twist (2X UAS-twist) in a wild-type background; (J-L) Embryos expressing one copy of UAS-twist or (M-O) embryos expressing two copies of UAS-twist in a da heterozygous background. Increasing Twist levels in the mesoderm while decreasing Da levels by 50% led to an increase of ectopic somatic muscle throughout the embryo measured by Mhc staining, shown here in ventral view (A,D,G,J,M), as well as a decrease in Fas III0-positive visceral muscle progenitors (B,E,H,K,N) and Zfh1-positive pericardial and cardial cells (C,F,I,L,O).