Fig. 3. The symmetric mitosis of GMC-1 and GMC1-1a in sli mutants. Embryos in A-F are double stained for Eve (red) and Zfh-1 (green; yellow indicates co-localization); G-I are double stained for Eve (red) and 22C10 (green). One segment per panel is shown, vertical lines indicate the midline. RP2 is indicated by large arrow, sib by small arrow, aCC by large arrowhead and pCC by small arrowhead. Scale bars, 15 µm. Anterior is up. (A,B) Wild-type embryos. The Eve-positive sib and pCC are Zfh-1-negative whereas both RP2 and aCC are Eve and Zfh-1 positive. (C,D) sli mutant embryos. The duplication of the RP2 occurs at the expense of the sib (hemisegments on the left). (E,F) sli mutant embryos showing the duplication of the aCC neurons at the expense of pCC (hemisegments on the right). (G) Wild-type embryo. Note the ipsilateral RP2 and posterolateral aCC projections (long arrows). (H,I) sli mutant embryos showing the duplication of the RP2 and aCC neurons at the expense of the sib and the pCC, respectively.