Fig. 4. The relationship between sli, insc and nb. Embryos in A-D are double stained for Eve (green) and Insc (red) and in E and F, for Eve. A-D show one hemisegment each; E and F, one segment each. Anterior is up, vertical lines mark the midline. Scale bars, 10 µm. (A,C) Wild-type embryos, Insc is asymmetrically localized (arrowhead) in GMC-1 and GMC1-1a. (B,D) sli mutant embryos, the localization of Insc in GMC-1 and GMC1-1a is non-asymmetric. (E) nb mutant embryo. In nb both the daughters of GMC-1 adopt a sib fate (arrows). (F) sli, nb double mutant embryo; both the daughters of GMC-1 adopt a sib fate.