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Fig. 7. Role of Robo in GMC-1 asymmetric division. Only half segments are shown, vertical lines indicate the midline. Scale bars, 15 µm. (A,B) Embryos stained for Eve (purple, nuclear) and Robo (red, membrane). Robo is in GMC1-1a (A) and GMC-1 (B). (C) Eve-stained robo null embryo, the GMC-1 symmetrically divides into two RP2s. (D) robo null embryo stained for Eve (red) and Zfh-1 (green; yellow indicates co-localization). The symmetrical division of GMC-1 and GMC1-1a into two RP2s and two aCCs (arrowheads) is shown.