Fig. 3. Defects in K(10) protein expression in orb^mel ovaries. Wild-type and orb^mel mutant ovaries were stained with Orb and K(10) antibodies. The two panels at the top show a stage 9 wild-type egg chamber stained with Orb (left) or K(10) antibody. Orb protein can be detected in the oocyte cortex, while K(10) protein is concentrated primarily (though not exclusively) in the oocyte nucleus. The four lower panels show Orb and K(10) proteins in stage 9 and early stage 10 orb^mel egg chambers. Though the level of Orb protein in these two egg chambers is reduced compared with wild type, the extent of reduction is not great as seen in other mutant chambers (see, for example, Figs 1, 5). In the top orb^mel chamber, some residual K(10) protein can be seen around the oocyte nucleus. In the bottom orb^mel chamber there is little K(10) protein.