Fig. 6. Orb protein expression is increased in K(10); orb^mel double mutants. Ovary extracts were prepared from wild-type w¹, orb^mel and K(10); orb^mel females. The Orb protein level was then analyzed by western blotting. A representative blot is shown in this figure. The two bands seen in this blot correspond to the two Orb protein isoforms typically seen in wild-type and orb^mel mutant ovaries. To control for the total protein in the extracts, the blots were reprobed with antibodies against the snRNP protein Snf or Armadillo (data not shown). Using NIH Image, the ratio of Orb protein to the loading control, Snf or Armadillo was calculated for each extract, and then normalized to that in wild-type ovaries. In the experiment shown here, the ratio is 1=w¹; 0.3=K(10); orb^mel and 0.05=orb^mel. The ratios estimated for the double mutant ranged in different experiments from 0.3 to 0.5 and for orb^mel from 0.05 to 0.1.