Fig. 1. Distribution of Me31B protein. (A) A confocal micrograph of stage 6 egg chamber expressing GFP-Me31B (green). The egg chamber is stained with phalloidin (red). GFP-Me31B signals show cytoplasmic particles in both nurse cells and the oocyte. (B) Higher magnification to highlight a ring canal. GFP-Me31B particles appear to pass through ring canal. (C-F) Distribution of endogenous Me31B. Wild-type ovaries were immunostained with an affinity-purified anti-Me31B antibody. Endogenous Me31B also distributed in a granular pattern in the cytoplasm of nurse cells and oocytes. Note that the antibody failed to permeate well in nurse cells in stage 10 egg chamber (F). (G) Cleavage embryos immunostained with anti-Me31B antibody. Me31B is uniformly distributed. By the cellular blastoderm stage (H), Me31B signal becomes undetectable. Scale bar in B: 5 µm.