Fig. 1. BSN-CM is necessary for isolated ureteric bud branching morphogenesis. Phase contrast photomicrographs of isolated ureteric buds cultured for 14 days in the presence (B) or absence (C) of BSN-CM in the presence of 10% FCS, 125 ng/ml GDNF, and 250 ng/ml FGF1. In the presence of BSN-CM, the T-shaped ureteric bud (A) underwent extensive branching morphogenesis (B). In the absence of BSN-CM, no significant growth/morphogenesis was observed (C). Scale bar, 500 µm.