Fig. 8 Effect of exogenous pleiotrophin on UB morphology in whole kidney organ culture. Fluorescence photomicrographs of E13 rat kidneys cultured for 7 days in DMEM/F12 supplemented with 10% FCS in the absence (A, control) or presence of pleiotrophin (B, 2.5 µg/ml; C, 5 µg/ml). The UB was visualized E13 mouse with FITC-conjugated lectin from Dolichos biflorus. Scale bar, 500 µm.