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Fig. 4. Preparation of recombinant TC14-2 (rTC14-2) and rTC14-3. (A,B) Bacterial lysates after IPTG induction of GST fusion proteins. (C,D) Affinity purification of rTC14-2 and rTC14-3 after thrombin digestion. (E,F) Affinity chromatography of rTC14-2 (E) and rTC14-3 (F), using immobilized galactose. Elution profiles show that both rTC14-2 and rTC14-3 have calcium-dependent, galactose-binding activity.