Fig. 6. Expression of TC14-3 during the asexual life span of P. misakiensis. (A) Blots of crude extracts and immunostaining with anti-TC14-3 monoclonal antibody. Lane 1, young functional animals; lane 2, adult animals of budding stage; lane 3, growing buds; lane 4, 1-day developing buds; lane 5, 2-day developing buds; lane 6, 3-day developing buds. (B) Section of the body wall of adult animal. Mesenchymal cells are stained. Scale bar, 25 µm. (C) Section of the pharynx of adult animal. Mesenchymal cells are stained heavily, and the pharyngeal epithelium is stained faintly (arrow). Scale bar, 25 µm. (D) Whole-mount of growing bud. Arrows indicate the atrial epithelium. Scale bar, 250 µm. (E) Section of growing bud. Both mesenchymal cells and atrial epithelium are stained. Scale bar, 25 µm. (F) Whole mount of 1-day-developing bud. Signal becomes weak at the proximal (boxed) area where morphogenetic events are in progress. Scale bar, 1 mm. (G) Section of boxed area in F. Scale bar, 25 µm. ae, atrial epithelium; e, epidermis (white dashed lines); m, mesenchymal cell.